Expression (average raw Ct, x axis) is plotted versus Standard Deviation of average raw Ct (y axis) for all of the candidate control genes. qRT-PCR was performed on RNA samples from 20 multiple sclerosis (MS) patients and 20 age and gender matched controls. The average expression and its standard deviation was calculated for each gene for all of the patients combined (n = 40). (Ct = Cycle threshold).
Stamova et al. BMC Medical Genomics 2009 2:49 doi:10.1186/1755-8794-2-49