Table 2

Interacting Proteins

Entrez ID

Symbol

Gene Name

Significant ELMs Present


59

ACTA2

actin, alpha 2, smooth muscle, aorta

The localization of the HIV-1 reverse transcription complex to actin microfilaments is mediated by the interaction of a reverse transcription complex component (HIV-1 Matrix) with actin, but not vimentin (intermediate filaments) or tubulin (microtubules)


60

ACTB

Actin, beta

Eukaryotic beta-actin binds to either the large subunit (p66) of HIV-1 reverse transcriptase or to the HIV-1 Pol precursor polyprotein in vitro; this interaction is believed to be important for the secretion of HIV-1 virions


70

ACTC1

actin, alpha, cardiac muscle 1

The localization of the HIV-1 reverse transcription complex to actin microfilaments is mediated by the interaction of a reverse transcription complex component (HIV-1 Matrix) with actin, but not vimentin (intermediate filaments) or tubulin (microtubules)


1457

CSNK2A1

casein kinase 2, alpha 1

Casein kinase II phosphorylates HIV-1 RT p66 and p51 in human cells


3439, 3440, 3449

IFNA1, IFNA2, IFNA16

IFN-alpha interferes with the initiation of HIV-1 reverse transcription resulting in a significant reduction in the relative levels of HIV-1 proviral DNA


3458

IFNG

Interferon, gamma

Up-regulation of LMP7 by IFN-gamma enhances proteasomal degradation of HIV-1 RT and presentation of the VIYQYMDDL epitope derived from HIV-1 RT


4772, 4773

NFACT1, NFACT2

nuclear factor of activated T-cells

NFATc facilitates HIV-1 RT reverse transcription activity and enhances HIV-1 infectivity in human T cells


5286

PIK3C2A

phosphoinositide-3-kinase, class 2, alpha polypeptide

HIV-1 RT heterodimer expressed in bacteria can be phosphorylated in vitro by several purified mammalian protein kinases including auto-activated protein kinase (PK), CKII, cytosolic protamine kinase (CPK), myelin basic protein kinase 1 (MBPK1), and PRKC


5578, 5579, 5580, 5581, 5584, 5588, 5590

PRKCA, PRKCB1,

PRKCD,

PRKCE, PRKCI, PRKCQ, PRKCZ

HIV-1 RT heterodimer expressed in bacteria can be phosphorylated in vitro by several purified mammalian protein kinases including auto-activated protein kinase (PK), CKII, cytosolic protamine kinase (CPK), myelin basic protein kinase 1 (MBPK1), and PRKC


5594, 5604, 6300

MAPK1, MAP2K1, MAPK12

mitogen-activated protein kinase 1

MEK1 in HIV-1 producer cells is able to activate virion-associated MAPK in trans, and the activated MAPK facilitates efficient disengagement of the HIV-1 reverse transcription complex from the cell membrane and subsequent nuclear translocation


5696

PSMB8

proteasome subunit, beta type, 8

Up-regulation of LMP7 by IFN-gamma enhances proteasomal degradation of HIV-1 RT and presentation of the VIYQYMDDL epitope derived from HIV-1 RT


6117, 6118, 6119

RPA1, RPA2, RPA3

Replication protein A and HIV-1 nucleocapsid protein interfere with the strand displacement DNA synthesis of HIV-1 reverse transcriptase by binding to the displaced strand and keeping it away from the newly synthesized strand


7150

TOP1

topoisomerase (DNA) I

Topoisomerase I (topo I) enhances HIV-1 reverse transcriptase activity in vitro and this effect can be inhibited by the topo I-specific inhibitor camptothecin


7157

TP53

tumor protein p53 (Li-Fraumeni syndrome)

Tumor suppressor protein p53 displays 3' -> 5' exonuclease activity, and interaction of p53 with HIV-1 reverse transcriptase (RT) can provide a proofreading function for HIV-1 RT


10527

IPO7

importin 7

Importin 7, an import receptor for ribosomal proteins and histone H1, is involved in the active nuclear import of the intracellular HIV-1 reverse transcription complex (RTC) containing HIV-1 RT, IN, NC, MA, and Vpr


29935

RPA4

replication protein A4, 34 kDa

Replication protein A and HIV-1 nucleocapsid protein interfere with the strand displacement DNA synthesis of HIV-1 reverse transcriptase by binding to the displaced strand and keeping it away from the newly synthesized strand


50810

hepatoma-derived growth factor, related protein 3

Hepatoma-derived growth factor 2 (HRP2) restores salt-stripped HIV-1 preintegration complex (PIC) activity in vitro


60489

apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G

Vif-negative HIV-1 produced from 293T cells transiently expressing hA3G are impaired in early and late viral DNA production, and in viral infectivity, which are correlated with an inability of tRNA(3)(Lys) to prime reverse transcription


A table of human proteins from the NIAID HIV-1 interaction database which are known to interact with HIV-1 RT and expressing the drug response predicting ELMs

Dampier et al. BMC Medical Genomics 2009 2:47   doi:10.1186/1755-8794-2-47

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