Table 4

Comparison of the clinicopathological features of 30 CRC patients according to the presence of NDRG2 methylation

Number of CRC

with NDRG2 methylation (%)

Number of CRC

without NDRG2 methylation (%)

p value


Age (yrs):

Age <50

4 (13.3)

5 (16.6)

ns

Age >50

4 (13.3)

17 (56.6)

Gender

Male

4 (26.7)

11 (73.3)

ns

Female

4 (26.7)

11 (73.3)

CRC:

Familiar

2 (25.0)

6 (75.0)

ns

Sporadic

6 (27.3)

16 (72.7)

Tumour location:

Proximal colon

2 (22.2)

7 (77.7)

ns

Distal colon

6 (28.6)

15 (71.4)

AJCC stage:

0

0

1 (4.8)

ns*

I

1 (12.5)

2 (9.5)

IIa

1 (12.5)

7 (33.3)

IIIb

0

3 (14.3)

IV

6 (75.0)

8 (38.1)

< 0,05^


MSI status1

High

1 (25.0)

3 (75.0)

ns

Low

1 (33.4)

2 (66.7)

Stable

6 (27.3)

17 (72.7)


Fisher exact test (2-tail); *Pearson Chi-square; ^Z test only for IV AJCC' stage

1Microsatellite instability (MSI) was determined by the mobility shift of PCR products using CC-MSI kit (AB Analitica s.r.l., Padova, Italy, http://www.abanalitica.com webcite), that include the Bethesda panel microsatellite (BAT25, BAT26, D5S346, D17S250 and D2S123) and other four mononucleotide microsatellite loci (NR21, NR24, BAT40 and TGFβRII), in tumours. Tumours showing instability in four or more markers were classified as high MSI, those showing it in two marker as low MSI, and those showing no instability as microsatellite-stable.

Piepoli et al. BMC Medical Genomics 2009 2:11   doi:10.1186/1755-8794-2-11

Open Data