Table 1

QPCR assays. QPCR assays (Assays on Demand) were from Applied Biosystems (Palo Alto, CA). Reverse primers from each assay were used for the synthesis of gene-specific cDNAs. They were provided separately by Applied Biosystems. Three assays (IGBP5_short, IGBP5_medium, IGBP5_long) were designed manually.

AoD

Assay

Acc_Nr

AmpliconSize

Module


Hs00608023_m1

BCL2

NM_000633

81

Estrogen

Hs00221277_m1

CEGP1

NM_020974

64

Estrogen

Hs00174860_m1

ESR1

NM_000125

62

Estrogen

Hs00172183_m1

PGR

NM_000926

118

Estrogen

Hs00180450_m1

GRB7

NM_005310

70

Her2

Hs01001598_g1

HER2

NM_004448

55

Her2

Hs00952036_m1

CTSL2

NM_001333

72

Invasion

Hs00171829_m1

STMY3

NM_005940

66

Invasion

Hs01030097_m1

CCNB1

NM_031966

66

Proliferation

Hs01032443_m1

MKI67

NM_002417

66

Proliferation

Hs00231158_m1

MYBL2

NM_002466

81

Proliferation

Hs00269212_m1

STK15

NM_003600

85

Proliferation

Hs00153353_m1

SURV

NM_001168

93

Proliferation

Hs99999903_m1

ACTB

NM_001101

171

Reference

Hs00266705_g1

GAPDH

NM_002046

74

Reference

Hs99999908_m1

GUSB

NM_000181

81

Reference

Hs99999902_m1

RPLP0

NM_001002

105

Reference

Hs00174609_m1

TFRC

NM_003234

79

Reference

Hs00430290_m1

UBB

NM_018955

120

Reference

Hs01630490_s1

RPL7A

BX641050

84

Reference

Hs00817975_g1

RPS11

NM_001015

168

Reference

Hs01922548_s1

RPS23

NM_001025

90

Reference

Hs00185390_m1

BAG1

NM_004323

58

Hs00154355_m1

CD68

NM_001251

68

Hs01383449_s1

GSTM1

AY532925

65

(own design)

IGBP5_short

NM_000599

60

Test

(own design)

IGBP5_medium

NM_000599

109

Test

(own design)

IGBP5_long

NM_000599

147

Test


Oberli et al. BMC Medical Genomics 2008 1:9   doi:10.1186/1755-8794-1-9

Open Data