|
QPCR assays. QPCR assays (Assays on Demand) were from Applied Biosystems (Palo Alto, CA). Reverse primers from each assay were used for the synthesis of gene-specific cDNAs. They were provided separately by Applied Biosystems. Three assays (IGBP5_short, IGBP5_medium, IGBP5_long) were designed manually. |
||||
| AoD |
Assay |
Acc_Nr |
AmpliconSize |
Module |
|
|
||||
| Hs00608023_m1 |
BCL2 |
NM_000633 |
81 |
Estrogen |
| Hs00221277_m1 |
CEGP1 |
NM_020974 |
64 |
Estrogen |
| Hs00174860_m1 |
ESR1 |
NM_000125 |
62 |
Estrogen |
| Hs00172183_m1 |
PGR |
NM_000926 |
118 |
Estrogen |
| Hs00180450_m1 |
GRB7 |
NM_005310 |
70 |
Her2 |
| Hs01001598_g1 |
HER2 |
NM_004448 |
55 |
Her2 |
| Hs00952036_m1 |
CTSL2 |
NM_001333 |
72 |
Invasion |
| Hs00171829_m1 |
STMY3 |
NM_005940 |
66 |
Invasion |
| Hs01030097_m1 |
CCNB1 |
NM_031966 |
66 |
Proliferation |
| Hs01032443_m1 |
MKI67 |
NM_002417 |
66 |
Proliferation |
| Hs00231158_m1 |
MYBL2 |
NM_002466 |
81 |
Proliferation |
| Hs00269212_m1 |
STK15 |
NM_003600 |
85 |
Proliferation |
| Hs00153353_m1 |
SURV |
NM_001168 |
93 |
Proliferation |
| Hs99999903_m1 |
ACTB |
NM_001101 |
171 |
Reference |
| Hs00266705_g1 |
GAPDH |
NM_002046 |
74 |
Reference |
| Hs99999908_m1 |
GUSB |
NM_000181 |
81 |
Reference |
| Hs99999902_m1 |
RPLP0 |
NM_001002 |
105 |
Reference |
| Hs00174609_m1 |
TFRC |
NM_003234 |
79 |
Reference |
| Hs00430290_m1 |
UBB |
NM_018955 |
120 |
Reference |
| Hs01630490_s1 |
RPL7A |
BX641050 |
84 |
Reference |
| Hs00817975_g1 |
RPS11 |
NM_001015 |
168 |
Reference |
| Hs01922548_s1 |
RPS23 |
NM_001025 |
90 |
Reference |
| Hs00185390_m1 |
BAG1 |
NM_004323 |
58 |
|
| Hs00154355_m1 |
CD68 |
NM_001251 |
68 |
|
| Hs01383449_s1 |
GSTM1 |
AY532925 |
65 |
|
| (own design) |
IGBP5_short |
NM_000599 |
60 |
Test |
| (own design) |
IGBP5_medium |
NM_000599 |
109 |
Test |
| (own design) |
IGBP5_long |
NM_000599 |
147 |
Test |
Oberli et al. BMC Medical Genomics 2008 1:9 doi:10.1186/1755-8794-1-9 |
||||