RNA isolation and characterization. Total RNA was isolated from kryo-sections (lanes A) and from paraffin sections according to the RNeasy FFPE protocol of Qiagen (lanes B), the ncLysis protocol of Applied Biosystems (lanes C) or according to our own protocol (lanes D). Aliquots of each RNA were separated by capillary electrophoresis (Agilent Bioanalyzer) on Nano chips along with RNA ladder (L; Ambion). Shown are RNAs from two representative tumors (Tu#10 and #18).
Oberli et al. BMC Medical Genomics 2008 1:9 doi:10.1186/1755-8794-1-9