SNPlex genotyping system assay protocol. On the first day, the OLA reaction, exonuclease purification, and PCR amplification are performed. On the second day, the amplicons are immobilized on streptavidin-coated microtiter plates. ZipChute probes are hybridized to complementary ZipCode sequences, and non-hybridized ZipChute probes are washed away. The bound ZipChute probes are eluted and analyzed by capillary electrophoresis using Applied Biosystems 3730. Reproduced from Journal of Biomolecular Techniques (Reference ) with permission from Andreas R Tobler at Applied Biosystems.
Dai et al. BMC Medical Genomics 2008 1:24 doi:10.1186/1755-8794-1-24