Open Access Highly Accessed Research article

The role of the small intestine in the development of dietary fat-induced obesity and insulin resistance in C57BL/6J mice

Nicole JW de Wit12*, Hanneke Bosch-Vermeulen12, Philip J de Groot12, Guido JEJ Hooiveld12, Mechteld M Grootte Bromhaar12, Jenny Jansen12, Michael Müller12 and Roelof van der Meer123

Author Affiliations

1 Nutrition, Metabolism & Genomics Group, Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands

2 Nutrigenomics Consortium, TI Food & Nutrition, Wageningen, The Netherlands

3 NIZO Food Research, Ede, The Netherlands

For all author emails, please log on.

BMC Medical Genomics 2008, 1:14  doi:10.1186/1755-8794-1-14

Published: 6 May 2008

Additional files

Additional file 1:

Diet composition. Composition of the low-fat and high-fat diets that were used in the diet intervention study in C57BL/6J mice.

Format: DOC Size: 55KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 2:

Outline of the diet intervention study in C57BL/6J mice. After a 3-weeks acclimatization period on the low-fat purified (LF) diet, C57BL/6J mice were fed a LF or high-fat (HF) purified diet for 2, 4 and 8 weeks (n = 6 per diet group, per time point). Body weight was recorded weekly. After 7 weeks of diet intervention, an oral glucose tolerance test (OGTT) was performed on six mice per diet group. 'A' indicates that for both diets at week 2, 4 and 8, microarray analysis was performed on the proximal, middle and distal part of the small intestine (SI), using pooled RNA samples for each diet group.

Format: PDF Size: 4KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 3:

Primer sequences. Overview of primer sequences that were used for qPCR verification of dietary fat-induced differential gene expression in the small intestine of C57BL/6J mice.

Format: DOC Size: 45KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 4:

Genes showing a consistent differential expression in the small intestine of C57BL/6J mice in all weeks of diet intervention. List of genes with the most pronounced (fold changes < -3.0 and > +3.0 in at least one week of diet intervention) and consistent differential gene expression in the small intestine of C57BL/6J mice during diet high-fat intervention.

Format: DOC Size: 168KB Download file

This file can be viewed with: Microsoft Word Viewer

Open Data

Additional file 5:

Verification of microarray results in individual mice by qPCR analysis. For the proximal (A), middle (B) and distal part of the small intestine (C), five genes that were found to be differentially expressed by microarray analysis were randomly selected and their expression was validated in individual mouse samples by qPCR. The qPCR data are visualized as the mean expression of all individual mice per diet group per time point ± SE, relative to the expression on the LF diet at week 2, which was set to 1. Only the results of the 18S normalization are shown as they are similar to the results obtained for the cyclophilin A normalization. White and black bars represent gene expression on the low- and high-fat diet, respectively. # significant differential gene expression indicated by MAS 5.0. * p < 0.05 (two-tailed Student's t test).

Format: PDF Size: 24KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data