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This article is part of the supplement: São Paulo Advanced School of Comparative Oncology: Abstracts

Open Access Poster presentation

miR-205 is involved in metastatic potential of 21T series, a breast cancer progression model

Luiza Stankevicins12*, Ana Barat2, Yegor Vassetzky2 and Claudia Vitória de Moura Gallo1

Author Affiliations

1 Departamento de Genética, Universidade do Estado do Rio de Janeiro, Instituto de Biologia Roberto Alcantara Gomes, Rio de Janeiro, Brazil

2 Université Paris-Sud 11 CNRS UMR 8126 «Signalisation, noyaux et innovations en cancérologie», Institut de Cancérologie Gustave-Roussy, Université Paris-Sud 11, Villejuif, France

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BMC Proceedings 2013, 7(Suppl 2):P44  doi:10.1186/1753-6561-7-S2-P44


The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1753-6561/7/S2/P44


Published:4 April 2013

© 2013 Stankevicins et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Background

microRNA (miRNA) is a class of non-coding RNAs, which regulate gene expression at the post-transcriptional level. Many miRNAs have been implicated in several human cancers, as these regulatory molecules play important roles in key cellular processes, including cell proliferation, differentiation and response to DNA damage.

Methods

To gain insights into the mechanisms involved in breast cancer initiation and progression we conducted a miRNA global expression on 21T series. These cell lines represent an in vitro model of breast cancer progression comprising immortalized cell lines derived from the same patient diagnosed with Her-2 overexpressing ductal carcinoma. The set include a normal epithelia (16N), primary in situ ductal carcinoma (21PT and 21NT) and cells derived from pleural effusion of lung metastasis (21MT-1 and 21MT-2). To confirm microarray results, the expression of the most significantly altered miRNAs were checked by qPCR. Matrigel invasion assay was done to evaluate the migration capacity of 21Tcells and Her-2, ZEB-1 and e-cadherin protein levels were achieved by western blot.

Results

Analysis of 21T series revealed a significant downregulation of miR-205 together with an enrichment of its predicted target, the pro-metastatic factor ZEB-1 and the consequent reduction of e-cadherin levels in the invasive 21MT cells.

Conclusions

These molecular alterations, in special the downregulation of miR-205 in cancer cells, can participate on modulation of epithelial to mesenchymal transition and increase the metastatic potential on breast cancer.

Financial support

Faperj, CAPES, CNPq and Université Paris XI.