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This article is part of the supplement: Beyond the Genome 2012

Open Access Poster presentation

A fast solution to NGS library preparation with low nanogram DNA input

Pingfang Liu*, Gregory JS Lohman, Eric Cantor, Bradley W Langhorst, Erbay Yigit, Lynne M Apone, Daniela B Munafo, Christine Sumner, Fiona J Stewart, Thomas C Evans, Nicole M Nichols, Eileen T Dimalanta and Theodore B Davis

  • * Corresponding author: Pingfang Liu

Author affiliations

New England Biolabs, Ipswich, MA 01938, USA

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Citation and License

BMC Proceedings 2012, 6(Suppl 6):P26  doi:10.1186/1753-6561-6-S6-P26


The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1753-6561/6/S6/P26


Published:1 October 2012

© 2012 Liu et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Poster presentation

Next-generation sequencing (NGS) has significantly impacted human genetics, enabling a comprehensive characterization of human genome as well as better understanding of many genomic abnormalities. By delivering massive DNA sequences at unprecedented speed and cost, NGS promises to make personalized medicine a reality in the foreseeable future. To date, library construction with clinical samples has been a challenge, primarily due to the limited quantities of sample DNA available. To overcome this challenge, we have developed a fast library preparation method using novel NEBNext reagents and adaptors, including a new DNA polymerase that has been optimized to minimize GC bias. This method enables library construction from an amount of DNA as low as 5 ng, and can be used for both intact and fragmented DNA. Moreover, the workflow is compatible with multiple NGS platforms.