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This article is part of the supplement: Beyond the Genome 2012

Open Access Open Badges Poster presentation

A fast solution to NGS library preparation with low nanogram DNA input

Pingfang Liu*, Gregory JS Lohman, Eric Cantor, Bradley W Langhorst, Erbay Yigit, Lynne M Apone, Daniela B Munafo, Christine Sumner, Fiona J Stewart, Thomas C Evans, Nicole M Nichols, Eileen T Dimalanta and Theodore B Davis

  • * Corresponding author: Pingfang Liu

Author Affiliations

New England Biolabs, Ipswich, MA 01938, USA

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BMC Proceedings 2012, 6(Suppl 6):P26  doi:10.1186/1753-6561-6-S6-P26

The electronic version of this article is the complete one and can be found online at:

Published:1 October 2012

© 2012 Liu et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Poster presentation

Next-generation sequencing (NGS) has significantly impacted human genetics, enabling a comprehensive characterization of human genome as well as better understanding of many genomic abnormalities. By delivering massive DNA sequences at unprecedented speed and cost, NGS promises to make personalized medicine a reality in the foreseeable future. To date, library construction with clinical samples has been a challenge, primarily due to the limited quantities of sample DNA available. To overcome this challenge, we have developed a fast library preparation method using novel NEBNext reagents and adaptors, including a new DNA polymerase that has been optimized to minimize GC bias. This method enables library construction from an amount of DNA as low as 5 ng, and can be used for both intact and fragmented DNA. Moreover, the workflow is compatible with multiple NGS platforms.