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This article is part of the supplement: International Conference for Healthcare and Medical Students 2011

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Understanding the etiology of ventral body wall defects

H Ghorab1* and J Thompson2

  • * Corresponding author: H Ghorab

Author Affiliations

1 Royal College of Surgeons in Ireland-Bahrain

2 University College Dublin, Belfield, Dublin 4, Ireland

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BMC Proceedings 2012, 6(Suppl 4):O10  doi:10.1186/1753-6561-6-S4-O10

The electronic version of this article is the complete one and can be found online at:

Published:9 July 2012

© 2012 Ghorab and Thompson; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Ventral body wall (VBW) defects are observed in 1 in 3000 births. The main types of defects include body wall dysplasia, gastroschisis, omphalocele and primary thoracoabdominoschisis. There is little evidence suggesting that genetic changes are associated with these defects. An important number of these occur as a result of exposure to chemical pollutants or radiation. In this project chick embryos exposed to cadmium were used as a model of VBW defect. The project aimed to identify when the ventral wall in chicks is fully closed and to investigate the migration of different cells and tissues in normal control embryos and compare it to embryos exposed to cadmium.


Fertile eggs were collected from a local hatchery. The embryos were placed in an incubator set at 37 °C, cadmium was administered ex ovo. The embryos were then fixed in 4F1G solution for at least 24 hours, following, the embryos were processed in paraffin wax which is firm enough to support subsequent cutting. The blocks were sectioned and attached to slides using a microtome. Finally the slides were stained using hematoxylin and eosin stains, hematoxylin stains the nuclei in a blue/black color whereas eosin stains cell cytoplasm and most connective tissue fibers in shades of pink, orange and red.


Gross anatomical differences between the controls and cadmium treated embryos were present, such as protrusion of abdominal contents out of the abdomen as well as abnormal body axial development. Microscopic differences include a large notochord, an underdeveloped dermomyotome and a disorganized sclerotomal migration.


VBW defects are uncommon however if present they may lead to an increase in neonatal morbidity and less frequently mortality. We have shown through this project that cadmium induced gross and microscopic anatomical defects in chick embryos. These findings imply that vertebral malformation may occur due to abnormal development of the somites giving rise to body axial deformity, which may directly contribute to primary VBW defects. Additional research is warranted to demonstrate pathways, which may be involved in normal formation of the ventral body wall.