Email updates

Keep up to date with the latest news and content from BMC Proceedings and BioMed Central.

This article is part of the supplement: 22nd European Society for Animal Cell Technology (ESACT) Meeting on Cell Based Technologies

Open Access Meeting abstract

A novel peptide to enhance recombinant BMP-2 production in mammalian cell cultures

Aileen J Zhou1*, Cameron ML Clokie12 and Sean AF Peel12

Author Affiliations

1 Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, University of Toronto, Toronto, Ontario Canada, M5G 1G6

2 Induce Biologics Inc, Toronto, Ontario, Canada, M5R 3N8

For all author emails, please log on.

BMC Proceedings 2011, 5(Suppl 8):P96  doi:10.1186/1753-6561-5-S8-P96


The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1753-6561/5/S8/P96


Published:22 November 2011

© 2011 Zhou et al; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Background

Due to their osteoinductive properties, recombinant human bone morphogenetic proteins (rhBMPs) have been used successfully for bone regeneration and replacement. However, the yields rhBMPs yields in mammalian expression systems are very low, resulting in their high cost. BMPs are synthesized as a precursor, proBMP, which undergoes enzymatic cleavage by proprotein convertases (PCs) to form the mature BMP [1]. Furin, an enzyme of the PC family, has shown to cleave BMP-4 [2] and BMP-2 (Zhou et al., unpublished data). This study investigated the effect and mechanism of action of polyarginine furin inhibitor, IND-1, on rhBMP-2 production in mammalian cell lines overexpressing rhBMP-2.

Materials and methods

Two stable cell lines expressing the hBMP2 gene, CHO-BMP2 and HEK-BMP2, were cultured in the presence of IND-1 in short-term (24 h, multi-well) and long-term (two-month, perfusion flasks) cultures. The rhBMP-2 produced was characterized by Western blot and its activity assessed using the C2C12 cell-based assay. The amount of proBMP-2 and mature BMP-2 produced was quantified by ELISA. The mRNA level of BMP-2 and furin in cells treated with or without IND-1 was compared by real-time RT-PCR. Cellular uptake of IND-1 was estimated by measuring the fluorescence of cell lysates following incubation with FITC labeled IND-1. Cellular PC activity post IND-1 incubation was measured using the Boc-RVRR-AMC substrate. Furin-specific siRNA was used to knock down the furin expression in CHO-BMP2 cells and its effect on the rhBMP-2 production was determined.

Results

Stably transfected CHO-BMP2 cells secreted 36 kDa rhBMP-2 dimers that were biologically active. In 24 h cell cultures, IND-1 treated cells produced significantly greater amounts of proBMP-2 (≥ 10-fold, P < 0.001) and mature BMP-2 (≥ 3-fold, P < 0.001) in their conditioned medium (Figure 1). In long-term CHO-BMP2 culture, IND-1 continued to increase the yields of BMP-2 (≥ 50%) and proBMP-2 (≥ 2-fold) without affecting cell growth or viability.

thumbnailFigure 1. After 24 h incubation with or without IND-1, the amount of proBMP-2 and mature BMP-2 proteins in (A) CHO and (B) HEK conditioned media were measured by proBMP-2 and BMP-2 ELISA. Data are presented as mean ± SEM (n = 3 experiments) (**P <0.01, ***P <0.001).

IND-1 treatment had no effect on the mRNA level of BMP-2 and furin, indicating IND-1 affects rhBMP-2 yield post-transcriptionally. While IND-1 was taken up by the cells and inhibited PC activity when added directly to the cell lysates, cells cultured with IND-1 showed no changes in their PC activity at doses 50 times higher than required to affect BMP-2 yields. Furthermore, knockdown of furin at both the mRNA (≥ 80%, P < 0.001) and the protein level (≥ 70%, P < 0.001), did not affect rhBMP-2 yields. These results suggest that furin inhibition is most likely not the mechanism by which IND-1 enhances rhBMP-2 yields.

Conclusions

The addition of a novel peptide IND-1 to the cell culture medium significantly enhanced the yields of both pro- and mature BMP-2 in stably transfected CHO and HEK cell lines. These increases were sustainable over an extended time period with regular IND-1 treatments. However, the enhanced rhBMP-2 yield is unlikely due to the well-established role of polyarginines as furin inhibitors.

Acknowledgements

This work was supported by Oral and Maxillofacial Surgery Foundation of Canada and Induce Biologics Inc.

References

  1. Wozney JM, Rosen V, Celeste AJ, Mitsock LM, Whitter MJ, Kriz RW, Hewick RM, Wang EA: Novel regulators of bone-formation-Molecular clones and activities.

    Science 1988, 242:1528-1534. PubMed Abstract | Publisher Full Text OpenURL

  2. Cui Y, Jean F, Thomas G, Christian JL: BMP-4 is proteolytically activated by furin and/or PC6 during vertebrate embryonic development.

    EMBO J 1998, 17:4735-4743. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL