Meeting abstract
A fructose containing cell culture medium has the advantage of low lactate production and a small pH change, leading to cell and product stability. But, not all cell lines grow well in the medium, and the fructose transporter, GLUT5, is related to it [1]. Thus, we developed an efficient production system of recombinant proteins by metabolic control and co-expression with GLUT5 in a fructose-based medium [2]. In this report, the availability of the GLUT5 co-expression system was indicated in CHO-K1 and the human cell line, SC-01MFP [3].
As a model, an IgG and GLUT5 co-expression vector was constructed and transfected into cells. When the transfected CHO-K1 and SC-01MFP cells were cultured in the fructose-based medium, both IgG productions were increased up to about two-fold of that cultured in the glucose-based medium (Table 1). Our study may be useful for efficient production of recombinant proteins using the fructose-based cell culture. In particular, the production in SC-01MFP cells is valuable for functional analysis of recombinant proteins with a human glycosylation profile.
Table 1. Proliferation and IgG production in the fructose-based medium.
References
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Inoue Y, Kawahara H, Shirahata S, Sugimoto Y: Retinoic acid improves a hybridoma culture in a fructose-based medium by up-regulation of fructose incorporation via retinoid nuclear receptors.
Biosci Biotechnol Biochem 2006, 70:2248-2253. PubMed Abstract | Publisher Full Text
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Inoue Y, Tsukamoto Y, Yamanaka M, Nakamura S, Inoue A, Nishino N, Kawahara H: Efficient production of recombinant IgG by metabolic control and co-expression with GLUT5 in a fructose-based medium.
Cytotechnology 2010, 62:301-306. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
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Kawahara H: Human cell stains for protein production, provided by selecting strains with high intracellular protein and mutating with carcinogens.
UK Patent 2008.
GB2426523
