A fructose containing cell culture medium has the advantage of low lactate production and a small pH change, leading to cell and product stability. But, not all cell lines grow well in the medium, and the fructose transporter, GLUT5, is related to it . Thus, we developed an efficient production system of recombinant proteins by metabolic control and co-expression with GLUT5 in a fructose-based medium . In this report, the availability of the GLUT5 co-expression system was indicated in CHO-K1 and the human cell line, SC-01MFP .
As a model, an IgG and GLUT5 co-expression vector was constructed and transfected into cells. When the transfected CHO-K1 and SC-01MFP cells were cultured in the fructose-based medium, both IgG productions were increased up to about two-fold of that cultured in the glucose-based medium (Table 1). Our study may be useful for efficient production of recombinant proteins using the fructose-based cell culture. In particular, the production in SC-01MFP cells is valuable for functional analysis of recombinant proteins with a human glycosylation profile.
Table 1. Proliferation and IgG production in the fructose-based medium.
UK Patent 2008.