Table 1

Box-Behnken results. a Three levels of concentrations for each variable including a maximum (1), a minimum (-1) and a center point (0) were used. Values shown in parenthesis are concentrations employed for r-transferrin and r-insulin (mg/mL) and lipid mix (based on Synthechol® concentrations provided in X). b Cell density values presented are mean of duplicate runs in million cells/mL.

EXP N°

r-Insulina

r-Transferrina

Lipid Mixa

Max Cell densityb


Experimental

According to model


1

-1 (1)

-1 (1)

0 (1)

3,1

2,8

2

1 (20)

-1 (1)

0 (1)

5,5

5,4

3

-1(1)

1 (20)

0 (1)

3,7

3,8

4

1 (20)

1 (20)

0 (1)

3,3

3,7

5

-1 (1)

0 (10)

-1 (0.1)

3,1

3,1

6

1 (20)

0 (10)

-1 (0.1)

4,6

4,4

7

-1 (1)

0 (10)

1 (2)

2,0

2,3

8

1 (20)

0 (10)

1 (2)

3,6

3,6

9

0 (10)

-1 (1)

-1 (0.1)

3,2

3,6

10

0 (10)

1 (20)

-1 (0.1)

3,5

3,4

11

0 (10)

-1 (1)

1 (2)

2,8

2,9

12

0 (10)

1 (20)

1 (2)

2,8

2,4

13

0 (10)

0 (10)

0 (1)

4,9

4,5

13

0 (10)

0 (10)

0 (1)

4,9

4,5

13

0 (10)

0 (10)

0 (1)

3,8

4,5


Cervera et al. BMC Proceedings 2011 5(Suppl 8):P126   doi:10.1186/1753-6561-5-S8-P126

Open Data