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This article is part of the supplement: IUFRO Tree Biotechnology Conference 2011: From Genomes to Integration and Delivery

Open Access Poster presentation

Apparent coordination of isocitrate dehydrogenase and glutamate decarboxylase expression in early stages of tree development

Juan Jesús Molina-Rueda1, María Belén Pascual2, Lara Silvia Jiménez-Bermúdez3, Francisco Miguel Cánovas2 and Fernando Gallardo2*

Author Affiliations

1 Present address: Rutgers University, Dpt Biological Sciences, Newark, New Jersey, USA

2 Universidad de Málaga, Dpt. Biología Molecular y Bioquímica, Málaga, Spain

3 Universidad de Málaga, SCAI, Servicio de Cultivo de Plantas, Málaga, Spain

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BMC Proceedings 2011, 5(Suppl 7):P66  doi:10.1186/1753-6561-5-S7-P66

The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1753-6561/5/S7/P66


Published:13 September 2011

© 2011 Molina-Rueda et al; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Poster presentation

The biosynthesis of 2-oxoglutarate and glutamate are key steps in the biosynthesis of nitrogen compounds and plant development. The reaction catalyzed by cytosolic isoenzyme of NADP+-linked isocitrate dehydrogenase (IDH) is also considered as the main route in the production of 2-oxoglutarate. According to its expression pattern during development, IDH is also involved in other, yet unknown, processes [1,2]. In addition to the importance of glutamate in the biosynthesis of nitrogen compounds, glutamate also serves as precursor of GABA, a molecule that is currently considered as a signal in higher plants. GABA is produced by the action of glutamate decarboxylase (GAD), a cytosolic enzyme that is regulated by Ca2+/calmodulin and pH. In contrast to IDH, that it is encoded by just one gene in most of plant genomes [2], GAD is encoded by a small family of nuclear genes [3]. The expression of IDH and GAD has been investigated during the differentiation of hypocotyl and stem in tree species. Our results indicate a coordination of the expression of IDH and GAD in developmental processes suggesting a role for 2-oxoglutarate supply and GABA synthesis during early stages of organ differentiation in trees.

References

  1. Palomo J, Gallardo F, Suárez MF, Cánovas C: Purification and characterization of NADP+-linked isocitrate dehydrogenase from Scots pine.

    Plant Physiol 1998, 118:617-626. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  2. Pascual MB, Molina-Rueda JJ, Cánovas FM, Gallardo F: Spatial distribution of cytosolic NADP+-isocitrate dehydrogenase in pine embryos and seedlings.

    Tree Physiol 2008, 28:1773-1782. PubMed Abstract | Publisher Full Text OpenURL

  3. Molina-Rueda JJ, Pascual MB, Cánovas FM, Gallardo F: Characterization and developmental expression of a glutamate decarboxylase from maritime pine.

    Planta 2010, 232:1471-1483. PubMed Abstract | Publisher Full Text OpenURL