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This article is part of the supplement: IUFRO Tree Biotechnology Conference 2011: From Genomes to Integration and Delivery

Open Access Poster presentation

Chemical screening for promotion of adventitious root formation in Eucalyptus globulus

Naoki Negishi*, Masatoshi Oishi and Akiyoshi Kawaoka

Author Affiliations

Nippon Paper Industries Co., LTD., Agri-Biotechnology Research Laboratory

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BMC Proceedings 2011, 5(Suppl 7):P139  doi:10.1186/1753-6561-5-S7-P139


The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1753-6561/5/S7/P139


Published:13 September 2011

© 2011 Negishi et al; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Poster presentation

Eucalyptus globulusis one of the most profitable trees for pulp and paper industries due to its fast growth and short harvesting cycle. This species is easily pulped with high yield, and its fiber qualities are among the best for paper production. However, it is difficult to vegetatively propagate E. globulus.So far, we have developed “photoautotropic culture method” that promotes rooting percentage by feeding approximately three times higher level CO2 level (1000 µmol mol–1) and suitable culture condition [1]. However, several lines showed poor rooting percentage even in the higher CO2 conditions. Therefore, it was necessary to develop novel method for promotion of adventitious root (AR) formation in E. globulus.

First, we measured endogenous levels of 20 kinds of hormones such as abscisic acid, auxins, cytokinines and gibberellins, at basal part of stem of easy-rooting line and poor-rooting line by UPLC-ESI-qMS/MS. As a result, the Indole-3-acetic acid (IAA) level of easy-rooting line was two times higher than that of the poor-rooting line, suggesting that endogenous IAA level may regulate ability of AR formation.

Next, we focused on the cytochrome P450s that are involved in a vast array of reactions of many different metabolic pathways. Several triazole-containing chemical compounds have previously been shown act as efficient inhibitors of cytochrome P450 monooxygenases. A chemical library of triazole derivatives to find chemicals which have the effect of promoting AR formation was screened. Consequently, five compounds effectively promoted AR formation.

Finally, we investigated how these chemicals affected the growth of Arabidopsis thaliana. Arabidopsis seedlings were grown on agar medium containing 1 μM selected chemicals. One of the selected chemicals, MA65 increased the number of roots in wild-type Arabidopsis seedlings and this phenotype was similar to a mutant superroot2 (sur2) [2]. The SUR2 gene encodes the cytochrome P450 CYP83B1, a modulator of auxin homeostasis. The amounts of endogenous IAA in 14-d-old Arabidopsis grown in the presence of 1 mM MA65 were analyzed. The IAA content was increased two-fold in the presence of MA65 as compared with untreated Arabidopsis. In addition, 1.0 cm explants of Arabidopsis stems were incubated for 7 d on MS medium containing 1 mMMA65. Stimulation of an AR formation was observed as compared to untreated samples. Taken together, MA65 may increase endogenous IAA level in a plant cell and promotes an AR formation.

Acknowledgements

We thank Dr Tadao Asami and Dr Hitoshi Sakakibara for technical help.

References

  1. Nagae S, Takamura T, Tanabe T, Murakami A, Murakami K, Tanaka M: In vitro shoot development of Eucalyptus citriodora on Rockwool in the film culture vessel under CO2 enrichment.

    J. Forest Research 1996, 1:227-230. Publisher Full Text OpenURL

  2. Delarue M, Prinsen E, Van Onckelen H, Caboche M, Ballini C: Sur2 mutations of Arabidopsis thaliana define a new locus involved in the control of auxin homeostasis.

    Plant J 1998, 14:603-611. PubMed Abstract | Publisher Full Text OpenURL