Email updates

Keep up to date with the latest news and content from BMC Proceedings and BioMed Central.

This article is part of the supplement: IUFRO Tree Biotechnology Conference 2011: From Genomes to Integration and Delivery

Open Access Poster presentation

Low temperature stress and changes in the lignin content and composition in Eucalyptus globulus

Jullyana Moura-Sobczak1*, Uiara Souza1, Eduardo Kiyota1 and Paulo Mazzafera2

Author Affiliations

1 1. Departamento de Fisiologia Vegetal. Instituto de Biologia - Unicamp. CEP: 13083-970 Campinas, SP, Brazil

2 4. Departamento de Fisiologia Vegetal. Instituto de Biologia - Unicamp. CEP: 13083-970 Campinas, SP, Brazil

For all author emails, please log on.

BMC Proceedings 2011, 5(Suppl 7):P102  doi:10.1186/1753-6561-5-S7-P102


The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1753-6561/5/S7/P102


Published:13 September 2011

© 2011 Moura-Sobczak et al; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Poster presentation

It is known that many abiotic stresses, such as mineral deficiency, drought, UV-B radiation, wind and low temperatures, alter the quantity and composition of lignin in several species [1]. The aim of this work was to verify if the cold stress may cause changes in the quantity and composition of lignin in E. globulus. Additionally, we analyzed whether these changes can also be beneficial from the industrial point of view, that is, if this would allow a better extractability of lignin in this species, which is a desirable feature in the manufacture of paper. During the day, all plants were kept in a greenhouse at room temperature but at night, half of plants was transferred to growth chamber at 12 °C and half to 25 °C. These conditions were kept during 20 days. The stems of plants from the two groups were collected and analyzed for total lignin with thioglycolic acid [2] and submitted to thioacidolysis and GC-MS analysis for the determination of the monomeric lignin composition [3]. Part of the material was used to determine the digestibility of cellulose [4].

It was observed that cold reduced the accumulation of lignin in E. globulus. GC-MS analysis showed that the proportion of the S/G was reduced in plants subjected to low temperatures and it was also observed a lower digestibility of cellulose in these plants, indicating that this lignin could be more difficult to be removed in industrial processes of papermaking.

These results have been related with gene expression studies for the enzymes of the lignin biosynthesis pathway and may contribute to understand the processes controlling lignin deposition in eucalyptus.

References

  1. Moura JCMS, Bonine CAV, Viana JOF, Dornelas MC, Mazzafera P: Abiotic and Biotic Stresses and Changes in the Lignin Content and Composition in Plants.

    J Integr Plant Biol 2010, 52:360-376. PubMed Abstract | Publisher Full Text OpenURL

  2. Lange BM, Lapierre C, Sandermann HJR: Elicitor-induced spruce stress lignin.

    Plant Physiol 1995, 108:1277-1287. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  3. Rolando C, Monties B, Lapierre C: Thioacidolysis. In Methods in Lignin Chemistry. Edited by Lin S, Dence C. Berlin: Springer-Verlag; 1992:334-349. OpenURL

  4. Chen F, Dixon RA: Lignin modification improves fermentable sugar yields for biofuel production.

    Nat Biotechnol 2007, 25:759-761. PubMed Abstract | Publisher Full Text OpenURL