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This article is part of the supplement: International Conference on Prevention & Infection Control (ICPIC 2011)

Open Access Poster presentation

Efficact of prion decontamination of medical instruments using alkaline and enzymatic detergents

A Sava1* and S Kritzler2

  • * Corresponding author: A Sava

Author Affiliations

1 Research, Novapharm Research, Sydney, Australia

2 Research, Novapharm Research Australia, Sydney, Australia

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BMC Proceedings 2011, 5(Suppl 6):P314  doi:10.1186/1753-6561-5-S6-P314

The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1753-6561/5/S6/P314


Published:29 June 2011

© 2011 Sava and Kritzler; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Introduction / objectives

The recent confirmation of Alzheimer’s disease transmissibility, together with the large amount of data regarding the infectious nature of prions and other protein misfolding diseases, highlights the need for reliable protein decontamination of all reusable medical instruments.

In order to decontaminate medical instruments of potentially infectious proteins to sterilisation assurance levels similar to current sterilisation techniques, the decontamination protocol should not only remove but, preferably, break up (hydrolyse) the infectious protein into small, water soluble fragments of less than 3-5 KDa.

Currently, the proteins are routinely removed during the instruments exposure to either alkaline or enzymatic detergents during washing.

Methods

Using straightforward stoichiometric equations and available literature data combined with our own results on kinetics of enzymatic and alkaline protein hydrolysis we assessed the quantities of dissolved and surface proteins that could be hydrolized during a standard CSDD wash cycle.

Results

We estimate that, during a common CSSD wash cycle, a formulated multi-enzymatic detergent with validated enzyme activity can cleave 200-1000 times greater number of peptide bonds than a comparable alkaline detergent.

We discuss numerous adjustments to the results that take into account protein folding, chemosorption on the wide spectrum of instrument surfaces, availability of cleaving sites, proteolysis resistance, etc.

Conclusion

A reliable removal of potentially infectious proteins and peptides from medical instruments is technically achievable via a standard 10-min, 60C CSSD wash with enzymatic detergents containing validated protein cleaving properties. The alkaline detergents, at realistic use levels, cannot assuredly hydrolyse proteins.

Disclosure of interest

None declared.