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This article is part of the supplement: International Conference on Prevention & Infection Control (ICPIC 2011)

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Is multi-drugs resistant Acinetobacter baumannii epidemic spread related to reduced susceptibility to biocides?

B Casini12*, MG Minacori1, A Buzzigoli1, P Valentini12, P Morici1, S Barnini3, C Tascini4, F Menichetti4, GM Rossolini5 and G Privitera12

  • * Corresponding author: B Casini

Author Affiliations

1 Dep. Experimental Pathology, MBIE, University of Pisa, Pisa, Italy

2 U.O. Igiene ed Epidemiologia, Azienda Ospedaliero-Universitaria Pisana, Pisa, Italy

3 U.O. Microbiologia, Azienda Ospedaliero-Universitaria Pisana, Pisa, Italy

4 U.O. Malattie Infettive, Azienda Ospedaliero-Universitaria Pisana, Pisa, Italy

5 Dep. Molecular Biology, University of Siena, Siena, Italy

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BMC Proceedings 2011, 5(Suppl 6):P300  doi:10.1186/1753-6561-5-S6-P300

The electronic version of this article is the complete one and can be found online at:

Published:29 June 2011

© 2011 Casini et al; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Introduction / objectives

Aim of this study was to assess the role of active extrusion in mediating decreased susceptibility to biocides in the persistance and spread of multi-drugs resistant of A. baumannii strains isolated in intensive care units of a tertiary-care teaching hospital.


67 clinical and 24 environmental strains isolated from 2007 to 2010 were genotyped by PFGE, MLST and REP-PCR. Multiplex PCRs were performed for identification of the ompA, csuE and blaOXA-51like sequence type groups. The antimicrobial susceptibility was determined and the presence of carbapenemase-encoding genes was analysed by characterization of the blaOXA genes. Chlorine susceptibility was analysed according to BS EN 1040:1997


The cross-analysis of genotyping methods allowed to group strains into 4 clones, only the clone A belonging to the Group 1 corrisponding to European II clonal complex and the clone B belonging to Group 2 clonal complex European I. Since 2008, a new variant of clone A has emerged as the predominant clinical and environmental strain, resulting positive for the presence of the carbapenemase OXA-58 plasmid-mediated and showing reduced chlorine susceptibility in vitro


Multi-antibiotic resistance may not be the only strategy applied by A. baumannii to spread and persist in healthcare setting, but also the increased resistance to biocides

Disclosure of interest

None declared.