Email updates

Keep up to date with the latest news and content from BMC Proceedings and BioMed Central.

This article is part of the supplement: Abstracts of the 16th International Charles Heidelberger Symposium on Cancer Research

Open Access Poster presentation

miR-143 over-expression reduces the growth of xenograft tumors from colon carcinoma cells

Pedro M Borralho1*, Sofia Gomes1, Raquel T Lima23, Rui E Castro1, Maria H Vasconcelos23 and Cecília MP Rodrigues1

Author Affiliations

1 Research Institute for Medicines and Pharmaceutical Sciences (iMed.UL), Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal

2 IPATIMUP, Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal

3 Department of Biological Sciences, Faculty of Pharmacy, University of Porto, Porto, Portugal

For all author emails, please log on.

BMC Proceedings 2010, 4(Suppl 2):P59  doi:10.1186/1753-6561-4-S2-P59


The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1753-6561/4/S2/P59


Published:24 September 2010

© 2010 Borralho et al; licensee BioMed Central Ltd.

Poster presentation

We have previously shown that miR-143 is down-regulated in colorectal cancer and that miR-143 over-expression in HCT116 cells increases sensitivity to 5-fluorouracil, reduces cell viability and increases apoptosis in vitro. In the present study, we evaluated the role of miR-143 over-expression on HCT116 xenograft tumor growth in nude mice. HCT116 cells with stable miR-143 over-expression (over-143) and control (empty) cells were injected s.c. into the backs of nude mice, and tumor growth was evaluated. Tumors arose approximately 14 days later, and the experiment was ended 40 days after injection. miR-143 was confirmed to be significantly over-expressed in over-143 versus empty xenografts, by Taqman real-time PCR. Over-143 xenografts displayed slower tumor growth compared to empty xenografts, with significantly smaller tumor volumes, from 23 until 40 days in vivo (p < 0.05), with final volumes of 928 ± 338 and 2312 ± 387 mm3, respectively. Evaluation of apoptotic proteins showed that over-143 versus empty xenografts, display reduced Bcl-2 expression, and increased caspase-3 activation and PARP cleavage (p < 0.05). In addition, the incidence of apoptotic cells, assessed by TUNEL, was increased in over-143 versus empty xenografts. Therefore, our results suggest that the reduced tumor volume may, in part, be due to increased miR-143-induced apoptosis. Collectively, our results reinforce the relevance of miR-143 in colorectal cancer, suggesting an important role in the control of in vivo tumor progression. This further expands its anti-proliferative, pro-apoptotic and chemosensitizer role that we have previously demonstrated in vitro.

Acknowledgements

This work was supported by PTDC/SAU-GMG/099162/2008, FCT, Portugal.