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This article is part of the supplement: Abstracts of the 16th International Charles Heidelberger Symposium on Cancer Research

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Dll4 blockade interferes with the bone marrow vascular niche and perturbs hematopoietic recovery following myeloablation

Leonor Remédio123*, Tânia Carvalho123 and Sérgio Dias123

Author Affiliations

1 Angiogenesis Lab, CIPM, Portuguese Institute of Oncology, IPOFG, EPE, Lisbon, Portugal

2 CEDOC, FCM, Universidade Nova de Lisboa, Lisbon, Portugal

3 Instituto Gulbenkian de Ciência, Oeiras, Portugal

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BMC Proceedings 2010, 4(Suppl 2):P38  doi:10.1186/1753-6561-4-S2-P38

The electronic version of this article is the complete one and can be found online at:

Published:24 September 2010

© 2010 Remédio et al; licensee BioMed Central Ltd.

Poster presentation

The Delta:Notch signaling pathway regulates numerous aspects of differentiation, morphogenesis and cell function, during embryogenesis and throughout adult life. More recently, Delta-like 4 (Dll4) has been shown to regulate adult neo-vascularization (angiogenesis). Consequently, therapeutic strategies to target Dll4 have been put forward for the treatment of different cancers. In the present study, we describe for the first time that Dll4 blockade affects the bone marrow (BM) microenvironment, by perturbing the BM vascular niche. Anti-Dll4 treatment administered 1 day after myeloablation increased the BM vascular content (CD31+ cells), promoted megakaryopoiesis and promoted hematopoietic (Sca1+) and endothelial progenitor (Flk1+CXCR4+) cell mobilization to the peripheral blood (PB). Moreover, anti-Dll4 treatment increased BM myeloid content, and induced mobilization of B (B220hiCD19lo) and T (CD3+NK1.1-) lymphocytes to the PB. Taken together, we show that Dll4 blockade affects the BM vascular niche, and as such affects hematopoietic cell differentiation and mobilization.