Determination of Km and Vmax for the 1MLGTG-RRR24 oligopeptide, based on the generation of acetylated 1MLGTG-RRR24 oligopeptide. Purified MBP-hNaa30p (80 nM) was incubated with 300 μM acetyl CoA and varying concentrations of 1MLGTG-RRR24 (30 – 350 μM) in acetylation buffer for 30 minutes at 37°C. A; Non linear regression analysis of the dose dependent curve generated based on the analysis of the acetylated oligopeptide absorption signal at 215 nm. The coefficient of determination (R2) is given above the plot. B; Hanes-Woolf plot of the dose dependent acetylation signal.
Evjenth et al. BMC Proceedings 2009 3(Suppl 6):S5 doi:10.1186/1753-6561-3-S6-S5