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This article is part of the supplement: Infectious diseases of the nervous system: pathogenesis and worldwide impact

Open Access Poster presentation

Early antibiotic administration prevents cognitive damage induced by pneumococcal meningitis in Wistar rats

Tatiana Barichello1*, Clarissa M Comim2, Geruza Z Silva1, Andréa C Ramos1, Ana Lúcia S Batista1, Geovana D Savi1, Gustavo Feier2, Felipe Dal-Pizzol1 and João Quevedo2

Author Affiliations

1 Laboratório de Fisiopatologia Experimental, Universidade do Extremo Sul Catarinense, 88806-000 Criciúma, SC, Brazil

2 Laboratório de Neurociências, Programa de Pós-Graduação em Ciências da Saúde, Unidade Acadêmica de Ciências da Saúde, Universidade do Extremo Sul Catarinense, 88806-000 Criciúma, SC, Brazil

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BMC Proceedings 2008, 2(Suppl 1):P5  doi:

The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1753-6561/2/S1/P5


Published:23 September 2008

© 2008 Barichello et al; licensee BioMed Central Ltd.

Poster presentation

Bacterial meningitis is an infection of the central nervous system characterized by a strong inflammation of the meninges and the subarachnoid space. Pneumococcal meningitis in humans is associated with long-term sequelae including sensory-motor deficits, seizures, and impairments of learning and memory. In order to evaluate this in an animal model, Streptococcus pneumoniae was cultured overnight in Todd Hewitt broth, diluted in fresh medium and grown to logarithmic phase, washed and resuspended in sterile normal saline 5 × 109 cfu/ml. Meningitis was induced by inoculating 10 μL of the S. pneumoniae suspension into the cisterna magna of the animals (rats, 60 days old, weighing 250–300 g) after removal of 10 μL of cerebrospinal fluid (CSF). All surgical procedures and bacteria administrations were performed under anesthesia. Meningitis was documented by a quantitative culture of 5 μl of CSF obtained by puncture of the cisterna magna at 8 h and 16 h after infection followed by the initiation of the antibiotic treatment (ceftriaxone 100 mg/kg bid). On day 10, rats were submitted to a behavioural task. Habituation to an open field was carried out in an open arena divided into 9 equal rectangles by black lines. Animals were gently placed on the left quadrant, and was allowed to explore the arena for 5 min (training session) and 24 hrs later submitted again to a similar session (test session). Crossing of the black lines and rearing performed in both sessions were counted. All data are presented as mean ± SD. Data were analyzed by Student's T test, considered p < 0.05 to be significant. In the rats that were treated with antibiotic beginning at both 8 h and 16 h after infection no differences in the number of crossings and rearings were observed between groups in the habituation to the open-field training session (p > 0,05). In the test session, in animals that antibiotic starts 8 h after infection, we did not observe reduction in both crossings and rearings in meningitis survivors rats compared with sham (p > 0.05). However, in rats that antibiotic starts 16 h after infection, we observed a significant reduction in both crossings and rearings in meningitis survivors rats compared with sham (p < 0.05). In conclusion, early antibiotic administration (8 h after infection) prevents cognitive damage induced by pneumococcal meningitis in Wistar rats.

Financial Support

CNPq, FAPESC and UNESC.