Email updates

Keep up to date with the latest news and content from BMC Proceedings and BioMed Central.

This article is part of the supplement: Infectious diseases of the nervous system: pathogenesis and worldwide impact

Open Access Poster presentation

Effect of CCR5 receptor antagonist (MET-RANTES) on leukocyte-endothelium interactions in an experimental model of herpetic encephalitis

Márcia de Carvalho Vilela12*, Daniel Mansur3, Norinne Lacerda-Queiroz4, David Henrique Rodrigues4, Débora Guerra Amaral2, Graciela Lima3, Erna Kroon3, Marco Antônio Campos3, Mauro Martins Teixeira2 and Antônio Lúcio Teixeira12

Author Affiliations

1 Departamento de Clínica Médica, Faculdade de Medicina, UFMG, Belo Horizonte, Minas Gerais, 31270-901, Brazil

2 Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, UFMG, Belo Horizonte, Minas Gerais, 31270-901, Brazil

3 Departamento de Microbiologia, Instituto de Ciências Biológicas, UFMG, Belo Horizonte, Minas Gerais, 31270-901, Brazil

4 Departamento de Morfologia, Instituto de Ciências Biológicas, UFMG, Belo Horizonte, Minas Gerais, 31270-901, Brazil

For all author emails, please log on.

BMC Proceedings 2008, 2(Suppl 1):P10  doi:


The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1753-6561/2/S1/P10


Published:23 September 2008

© 2008 Vilela et al; licensee BioMed Central Ltd.

Background

The Herpes Simplex Virus-1 (HSV-1) is responsible for several clinical manifestations in humans, including severe encephalitis. The aim of this study was to use Met-RANTES, a CCR1 and CCR5 antagonist, in the treatment of a severe model of HSV-1 encephalitis.

Methods

Wild type C57BL/6 mice were intracerebrally inoculated with 104 PFU of HSV-1 or PBS. Mice received Met-RANTES (10 μg/mice) subcutaneously on days -1, 0, 1, 2 and 3. Visualization of leukocyte recruitment using intravital microscopy was done at 1 and 3 days post-infection (dpi). The brain was removed for chemokine analysis by ELISA. Virus titration in cerebral tissue was done by TCID50.

Results

Met-RANTES does not alter significantly virus titers at 1 dpi and 3 dpi after intracerebral injection of HSV-1. This treatment decreased leukocyte adhesion of microvasculature in C57BL/6 infected mice at 1 and 3 dpi. Treatment was also followed by a significant increase in chemokine levels, including CCL3, CCL5, CXCL1 and CXCL9, at 3 dpi.

Conclusion

CCL5/RANTES, a chemokine that binds to CCR1 and CCR5, seems to be relevant in the recruitment of leucocytes in an experimental model of severe HSV-1 encephalitis. However blocking of CCR1 and CCR5 does not affect HSV-1 replication, suggesting that other immune mechanisms are involved in this process.