Resolution:
## Figure 4.
Cell response distribution to autoinducer induction in the stochastic model. Cell response probability after 10 hours (top: A, B) and 100 hours (middle: C, D) of induction at different autoinducer concentrations for the lux01 (left: A, C) and lux02 (right: B, D) operons in the stochastic model. The distribution reveals the coexistence of two
subpopulations with low and high GFP expression when the cells are induced at intermediate
autoinducer concentrations. The region of bistability (precision) is defined by the
range of for which the response is bimodal according to the following criterion: the lower/upper
limit of the bistable region (orange lines) is defined by the value of for which 90% of the cells are in the low/high state. The black line stands for the
concentration of GFP (normalized) as a function of in the deterministic model at the steady state. After 10 hours of induction (top:
A, B) most cells are still in a transient state if . After 100 hours of induction (middle: C, D), the bimodality region shrinks and the precision increases. The population average
curves of the induction and dilution experiments in the stochastic model (bottom:
E, F, dashed lines) show that the intrinsic noise allows cells to jump to the high state
inside the deterministic bistable region. On the other hand, the transition from high
to low follows the deterministic path thus indicating that the switching rate in this
case is close to zero.
Weber and Buceta |