Table 8

Synthetic lethality induced by elimination of phosphorylation/dephosphorylation reactions
Eliminated reaction Impacted single mutation strains that are
viable (inviable) before (after) perturbation
Whi5 phosphorylationby Bck2 cln3 Δ, Multicopy BCK2, cdh1 Δ, sic1 Δ, swi5 Δ,
CLB5-db Δ, net1-ts, GAL-CLB2, APC-A
CKI phosphorylation by Cln2 bck2 Δ, GAL-SIC1, net1-ts, APC-A
CKI phosphorylation by Clb2 GAL-CLN3, cdh1 Δ, GAL-CLB5, CLB1 clb2 Δ
CKI dephosphorylation by Cdc14 bck2 Δ, cdh1 Δ, GAL-CLB2, APC-A
Whi5 phosphorylation by Cln3 bck2 Δ, cdh1 Δ, APC-A
SBF phosphorylation by Clb2 cdh1 Δ, CLB5-db Δ, APC-A
Whi5 phosphorylation by Cln2 bck2 Δ,APC-A
Whi5 dephosphorylation by Cdc14 APC-A
Net1 dephosphorylation by PPX Multicopy CDC15

Upon setting phosphorylation/dephosphorylation rate constants to zero (left column), viability is lost in several single mutation strains (right column).

Oguz et al.

Oguz et al. BMC Systems Biology 2013 7:53   doi:10.1186/1752-0509-7-53

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