Table 2

Comparison of experimental observations with simulation
Experiment Phenotype Reference Model adaptation Simulation
reduced RsbX increase in β-Gal response [25] decrease in kdps increase in post-stress RsbS-P
ΔRsbR(AB), ΔRsbR(ABC), ΔRsbR(ABD) alteration in pre- and post-stress β-Gal response [13] increase in kphs increase in RsbS-P
ΔRsbR(ABC), RsbRD stressosome hyperbolic response [14] increase in background phosphorylation (allost. par.) decrease of cooperativity hyperbolic response
RsbR, RsbS phosphorylation after stimulus transient increase in phosphorylation level [20] increase in kphr increase of RsbR-P
stimulation with different stress level sigmoidal dose–response curve [10] adaptation of the allosteric parameter for R1 and R2 neighbours allosteric activation of RsbT by RsbR-P
RsbRA T86A, N129A, Q142A, etc. elevated basal β-Gal level but wild type stress response [31,32] increase of pre-stress kphr increase of pre-stress RsbR-P
RsbRA L141A, Q147A, L149R reduced basal β-Gal level but wild type stress response [32] decrease of pre-stress kphr decrease of pre-stress RsbR-P

The phosphorylation of RsbS is correlated to the β-galactosidase response because phosphorylated RsbS releases RsbT, the activator of σB.

Liebal et al.

Liebal et al. BMC Systems Biology 2013 7:3   doi:10.1186/1752-0509-7-3

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