Mapping result of hsa-mir-1307 reads in the SRX018957 library. Hsa-mir-1307 encodes mature miRNA hsa-miR-1307 at its 3p arm, ranging from nt 80 to 101 of pre-miRNA, as presented in uppercase. The sequence reads mapped to hsa-mir-1307 were arranged according to their mapped loci within the pre-miRNA. The expression level of a mature miRNA is determined by summing the read counts (initial read counts, not normalized as TPM, transcript per million) of all its iso-reads, isomiRs. The mismatches at the 3' ends, shown in lowercase, denoted the modified fragments. In the figure, only the modified fragments, including A, U, AA, AU and UU, with frequency 1% were recovered.
Li et al. BMC Systems Biology 2012 6(Suppl 2):S14 doi:10.1186/1752-0509-6-S2-S14