# Table 4

Constrained alignment translation error. Constraining the search to rotations around a single axis. Subtomogram alignment error in terms of the difference in the Euclidean distance between determined and true subtomogram translations. Shown are the medians and median absolute deviations of all 100 subtomogram alignments.

60° angle interval, RCS

SNR

1

0.5

1

Tilt

±90°

0.018 ± 0.0053

0.047 ± 0.017

0.08 ± 0.023

0.28 ± 0.1

±80°

0.027 ± 0.011

0.069 ± 0.029

0.1 ± 0.048

0.37 ± 0.17

±70°

0.037 ± 0.017

0.085 ± 0.04

0.13 ± 0.059

0.45 ± 0.28

± 60°

0.055 ± 0.028

0.14 ± 0.083

0.19 ± 0.11

0.59 ± 0.36

±50°

0.1 ± 0.067

0.18 ± 0.12

0.24 ± 0.12

0.74 ± 0.42

±40°

0.27 ± 0.25

0.49 ± 0.4

0.94 ± 0.83

3.3 ± 2.3

60° angle interval, FCS

SNR

1

0.5

0.1

Tilt

±90°

0.018 ± 0.0062

0.05 ± 0.017

0.074 ± 0.021

0.23 ± 0.097

±80°

0.027 ± 0.013

0.063 ± 0.025

0.076 ± 0.029

0.24 ± 0.091

±70°

0.034 ± 0.015

0.077 ± 0.032

0.098 ± 0.036

0.32 ± 0.16

±60°

0.055 ± 0.033

0.12 ± 0.063

0.18 ± 0.099

0.46 ± 0.28

±50°

0.11 ± 0.075

0.16 ± 0.099

0.2 ± 0.11

0.6 ± 0.36

±40°

0.39 ± 0.35

0.36 ± 0.27

0.57 ± 0.44

3.5 ± 2.8

45° angle interval, RCS

SNR

1

0.5

0.1

Tilt

±90°

0.015 ± 0.005

0.054 ± 0.014

0.063 ± 0.019

0.24 ± 0.086

±80°

0.024 ± 0.0072

0.056 ± 0.018

0.086 ± 0.028

0.29 ± 0.12

±70°

0.033 ± 0.0095

0.075 ± 0.028

0.11 ± 0.04

0.38 ± 0.17

±60°

0.046 ± 0.019

0.1 ± 0.036

0.16 ± 0.051

0.48 ± 0.22

±50°

0.067 ± 0.035

0.14 ± 0.06

0.22 ± 0.098

0.59 ± 0.27

±40°

0.22 ± 0.18

0.31 ± 0.21

0.44 ± 0.29

2.3 ± 1.7

45° angle interval, FCS

SNR

1

0.5

0.1

Tilt

±90°

0.017 ± 0.0053

0.048 ± 0.014

0.06 ± 0.016

0.21 ± 0.074

±80°

0.021 ± 0.0069

0.052 ± 0.018

0.073 ± 0.025

0.2 ± 0.067

±70°

0.03 ± 0.011

0.065 ± 0.023

0.098 ± 0.03

0.26 ± 0.085

±60°

0.043 ± 0.017

0.088 ± 0.033

0.13 ± 0.044

0.32 ± 0.11

±50°

0.07 ± 0.032

0.14 ± 0.056

0.18 ± 0.073

0.44 ± 0.19

±40°

0.17 ± 0.11

0.24 ± 0.16

0.33 ± 0.18

1.5 ± 1.2

Xu and Alber BMC Systems Biology 2012 6(Suppl 1):S18   doi:10.1186/1752-0509-6-S1-S18