Table 4

SrbA binding sites

Gene

Position

Strand

Sequence

ka(M-1s-1)

kd(s-1)

KD(nM)


srbA

-735 to -726

Sense

ATCATACGAT

1.48 ± 0.05 * 107

9.27 ± 0.29 * 10-3

0.63 ± 0.04

srbA*

-735 to -726

Sense

AT

    ATA
AC
    ATA

5.92 ± 0.08 * 105

2.29 ± 0.01* 10-1

386 ± 7.3

hapX

-774 to -783

Antisense

ATCCTCCCAT

5.68 ± 0.07 * 105

1.61 ± 0.01* 10-1

282 ± 5.1

hapX

-1340 to -1331

Sense

ATCAGATGAT

3.10 ± 0.01 * 107

1.44 ± 0.01 * 10-1

4.64 ± 0.03

hemA

-527 to -518

Sense

ATCGGATCAT

1.41 ± 0.07 * 107

5.88 ± 0.28 * 10-2

4.18 ± 0.41

hemA

-338 to -347

Antisense

ATCGCCTCAT

1.03 ± 0.01 * 106

3.66 ± 0.02 * 10-2

35.7 ± 0.52


The binding of SrbA to five predicted binding sites was analysed. Results indicate three high-affinity binding sites in promoter regions of hapX, hemA and srbA. The asterix denotes a mutated binding site. Mutated nucleotides are underlined. ka= association rate constant, kd = dissociation rate constant, KD = kd/ka equilibrium dissociation constant.

Linde et al. BMC Systems Biology 2012 6:6   doi:10.1186/1752-0509-6-6

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