Additional file 2.
TNFα dose-dependent induction of NF-κB nuclear translocation.(a) Non-treated NHBE cells show a diffuse cytoplasmic staining of NF-κB (left) while after adding TNFα to the culture medium (right), the nucleus of stimulated cells is strongly labeled (magnification 10X/0.3); (b) NF-κB nuclear fluorescence intensity (FI) per dose of TNFα. For each group, the nuclear fluorescence intensity was measured in 500 cells per well of three replicates. Across-group comparisons by one-way ANOVA test were all p < 0.001.
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Martin et al. BMC Systems Biology 2012 6:54 doi:10.1186/1752-0509-6-54