Figure 8.

Transient times to approach steady-state for the basic and extended model. Parameter values for the basic model: λ₋₁ = 10 s⁻¹,λ₁ = 1μM⁻¹s⁻¹,k₁ = 1 s⁻¹,λ₋₂ = 10 s⁻¹,λ₂ = 20μM⁻¹s⁻¹,k₂ = 1 s⁻¹,P_t = 1μM,S_t = 10μM.Parameter values for the extended model: λ₋₁ = 10 s⁻¹,λ₁ = 1μM⁻¹s⁻¹,k₁ = 1 s⁻¹,λ₋₂ = 10 s⁻¹,λ₂ = 20μM⁻¹s⁻¹,k₂ = 1 s⁻¹,κ₋₁ = 50 s⁻¹,κ₁ = 1μM⁻¹s⁻¹,λ₋₃ = 10 s⁻¹,λ₃ = 1μM⁻¹s⁻¹,k₃ = 100 s⁻¹,P_t = 1μM,S_t = 10μM.The transient times for R = ([S_p] + [S_pP] + [S_pPK])/S_t were estimated such that the absolute difference between the positive steady-state and the time solution at the transient time is within a small tolerance and that the transient times do not change too much if the tolerance is varied. Hence, we chose the tolerance to be 10⁻⁴.