Table 1

Thermodynamic equilibrium constants (Keq) of selected glycolytic reactions at 12 and 30°C
Enzyme Abrev. Keq Fold change
12°C 30°C
Hexokinase HXK 7.7 x103 3.5 x103 0.45
Phosphoglucose isomerase PGI 0.27 0.29 −0.93
Phosphofructokinase PFK 5.0 x103 2.4 x103 2.08
Fructose-1,6-biphosphate aldolase FBA 9.3 x10-4 1.4 x10-3 −1.51
Triosphosphate isomerase TPI 0.040 0.048 −1.20
Glyceraldehyde-3-phosphate dehydrogenase TDH 0.66 0.69 −1.05
Phosphoglycerate kinase PGK 20 16 1.25
Phosphoglycerate mutase PGM 0.074 0.087 −1.18
Enolase ENO 5.1 4.5 1.13
Pyruvate kinase PYK 3.9 x104 1.4 x104 2.79

Keq values were calculated from [24] considering a reference condition of 298.15 K, I = 0.25M and pH 7.0. The fold change refers to the ratio Keq 12°C/Keq 30°C.

Cruz et al.

Cruz et al. BMC Systems Biology 2012 6:151   doi:10.1186/1752-0509-6-151

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