Simulation results of intracellular metabolite levels, normalized to the levels under glucose-excess conditions at 30°C. (A) kcat of glyceraldehyde 3-phosphate dehydrogenase (TDH) was set to be 2 times less sensitive to temperature than the other glycolytic enzymes; (B) all kcat were set to have the same temperature dependency. The symbols refer to simulations of: sinoidal temperature cycles (△), linear temperature shifts applied to 30°C steady-state chemostats (○) and batch fermentations at different temperatures (□). The colors indicate the culture temperature at the time of sampling. Simulation results considering different temperature sensitivities for phosphofructokinase and pyruvate kinase can be found in the Additional file 1: Figures S1A and B.
Cruz et al. BMC Systems Biology 2012 6:151 doi:10.1186/1752-0509-6-151