Open Access Methodology article

Flux variability scanning based on enforced objective flux for identifying gene amplification targets

Jong Myoung Park12, Hye Min Park1, Won Jun Kim1, Hyun Uk Kim12, Tae Yong Kim12 and Sang Yup Lee123*

Author Affiliations

1 Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 305-701, Republic of Korea

2 BioInformatics Research Center, KAIST, Daejeon, 305-701, Republic of Korea

3 Department of Bio and Brain Engineering and BioProcess Engineering Research Center, KAIST, Daejeon, 305-701, Republic of Korea

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BMC Systems Biology 2012, 6:106  doi:10.1186/1752-0509-6-106

Published: 21 August 2012

Additional files

Additional file 1:

Bacterial strains and plasmids used in this study. (PDF 142 kb)

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Additional file 2:

Oligonucleotides used in this study. (PDF 101 kb)

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Additional file 3:

Genomic context and flux-converging pattern analyses for shikimic acid and putrescine production inEscherichia coli. (PDF 143 kb)

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Additional file 4:

Analysis of flux patterns with partial variations. (PDF 84 kb)

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Additional file 5:

Putrescine production yield (g putrescine/g glucose) for the single gene-overexpressing strains based onE. coliXQ52 (p15SpeC) strain by flask cultivation on R/2 medium supplemented with 10 g/L glucose at 37 °C. (PDF 147 kb)

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