Pipeline. We compared the protein profiles of six cervical cancer cell lines by 2D SDS-PAGE, and established a set of proteins common to these cell lines, and which did not feature in our control. This set of proteins was termed "central core of cervical cancer", (a). From the central core, we reconstructed a PPI network (b), this network expanded and used to obtain the overrepresented GO's and pathways. Finally, we conducted an analysis of the transcription factors contained in the extended network using ChIP-Seq analysis of the ENCODE project (c).
Higareda-Almaraz et al. BMC Systems Biology 2011 5:96 doi:10.1186/1752-0509-5-96