Expression variability is increased for genes interacting with the aneuploid chromosome under conditions of rapid cell division. The variability of mRNA expression level (absolute value of fold change) was used as a predictor of the presence of a protein interaction using logistic regression. Three models were tested. The first two predict protein interaction presence (i.e., membership in the set PPI) using the basal mRNA expression variation from either the control (Ctrls, no aneuploid chromosome; solid lines) or the experimental (Expts, presence of an aneuploid chromosome; dashed lines) strains. The third uses expression data to predict the presence of an interaction with a protein encoded on an aneuploid chromosome (i.e., membership in AneuPPI; dotted lines), given that the gene in question is already a member of PPI. (A) Expression measured under rapid growth conditions (batch culture). (B) Expression measured under slow growth conditions (chemostat, phosphate-limited).
Bekaert and Conant BMC Systems Biology 2011 5:62 doi:10.1186/1752-0509-5-62