Figure 4.

All scenarios of the model are compatible with previous measurements and predictions. A: Variation in the average number of RNAP molecules per cell with doubling time. Simulations from the three scenarios (connected triangles, squares and crosses) are compared to the (validated) predictions of ref. [41]. Scenarios 1b and 2 are compatible with the results (which are assumed in scenario 1a). B: Variation in the average expression rate per cell of the dnaA gene with growth rate in the three scenarios (connected triangles, squares and crosses) agrees with our direct measurements (Chiara Saggioro, Anne Olliver, Bianca Sclavi: Multiple levels of regulation in the growth rate dependence of DnaA expression, submitted). The measurements (pentagons) are obtained with GFP reporters on a plasmid, normalized by plasmid number and gene copy-number with varying growth rate. The experiment details are available in Additional File 1, Section 7. This prediction is also compatible with the results of Chiaramello and Zyskind [20].

Grant et al. BMC Systems Biology 2011 5:201   doi:10.1186/1752-0509-5-201
Download authors' original image