14-3-3 proteins interact with TCA cycle enzymes in yeast. Three 14-3-3 isoforms interact with isocitrate dehydrogenase (ICDH, At4G35650) and two malate dehydrogenases (MDH1, At1G04410; MDH2, At5G43330) that were previously isolated as possible targets of 14-3-3 proteins. AC indicates a pGADT7 and BD indicates a pGBKT7 vector. The positive control and negative control were described in methods section.
Diaz et al. BMC Systems Biology 2011 5:192 doi:10.1186/1752-0509-5-192