Figure 2.

Workflow for building the cell-specific reconstructions. Human Recon 1 [5] was utilized as the starting point for modeling human metabolism. The genome sequence annotation upon which Recon 1 was built was updated, and all incorrectly lumped and balanced reactions were corrected. Multiple reaction and enzyme databases, including UniProt, were then used to build a draft model. The draft model was then manually curated using literature and multiple high-throughput data sets. In addition, important metabolic pathways that were not in Recon 1 (e.g. hepatic ketogenesis) were added accordingly to the cell-specific reconstructions.

Bordbar et al. BMC Systems Biology 2011 5:180   doi:10.1186/1752-0509-5-180
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