Table 2

Isotopologue distribution produced by isolated hepatocytes in the presence of glucose and lactate.

Experiment 1

Simulations

Experiment 2

Simulations

label in glucose

B

A

label in lactate

B

A


glucose:

χ2 = 7.21

5.72

χ2 = 5.52

0.576

m0

0.532 ± 0.0098

0.514

0.514

0.979 ± 0.01

0.976

0.984

m1

0.00846 ± 0.0022

0.0114

0.00918

0.0063 ± 0.0055

0.00484

0.00354

m2

0.459 ± 0.0103

0.473

0.475

0.0055 ± 0.0064

0.00891

0.00469

m3

-- --

--

--

0.0064 ± 0.0016

0.00996

0.00628

[mM]

20.6 ± 2.91

21

21.1

20.9 ± 2.22

20.8

20.9

glycogen:

χ2 = 6.46

27.3

χ2 = 2.22

5.73

m0

0.681 ± 0.032

0.683

0.57

0.909 ± 0.026

0.907

0.9

m1

0.0119 ± 0.031

0.00767

0.0131

0.017 ± 0.0066

0.0166

0.0225

m2

0.302 ± 0.031

0.308

0.409

0.038 ± 0.01

0.0313

0.0298

m3

0.0017 ± 0.001

0.000136

0.00208

0.0273 ± 0.0071

0.0363

0.04

m4

0.0032 ± 0.0016

0.000593

0.00565

0.0036 ± 0.0016

0.00329

0.00317

m5

-- --

--

--

0.003 ± 0.014

0.00335

0.00271

mg/mL

0.263 ± 0.084

0.256

0.196

0.262 ± 0.0691

0.256

0.196

glgn14:

χ2 = 5.31

19.2

χ2 = 2.23

4.59

m0

0.678 ± 0.032

0.692

0.588

0.93 ± 0.024

0.924

0.921

m1

0.016 ± 0.0046

0.00561

0.0112

0.033 ± 0.009

0.0343

0.0472

m2

0.3 ± 0.032

0.302

0.399

0.019 ± 0.0079

0.0178

0.0128

m3

-- --

--

--

0.014 ± 0.005

0.0209

0.0163

m4

-- --

--

--

0.004 ± 0.003

0.00267

0.0264

glgn36:

χ2 = 1.48

14.2

χ2 = 1.97

4.48

m0

0.98 ± 0.0101

0.987

0.968

0.924 ± 0.024

0.923

0.911

m1

0.00408 ± 0.0018

0.0051

0.0101

0.0265 ± 0.007

0.0332

0.0348

m2

0.0139 ± 0.0078

0.00766

0.216

0.027 ± 0.0081

0.0188

0.0219

m3

-- --

--

--

0.021 ± 0.0067

0.0221

0.0292

lactate:

χ2 = 2.06

2.73

χ2 = 1.62

9.76

m0

0.974 ± 0.026

0.991

0.985

0.636 ± 0.017

0.621

0.608

m1

0.0026 ± 0.0019

0.000974

0.00135

0.0166 ± 0.0025

0.0167

0.0172

m2

0.0094 ± 0.0037

0.00773

0.0141

0.0318 ± 0.0035

0.0302

0.0245

m3

0.00136 ± 0.023

0.00000227

0.0000436

0.316 ± 0.0213

0.332

0.35

[mM]

6.18 ± 0.75

6.8

6.73

3.18 ± 0.43

6.29

6.22

Σχ2

22.52

69.15

13.56

25.136


Before incubation the medium contained either 50% of [1,2-13C2]D-glucose and unlabeled lactate (experiment 1) or 50% uniformly 13C-labeled lactate and unlabeled glucose (experiment 2). The measurements are presented as mean ± standard deviation. The data were fit by two models (A and B). The conditions of incubation and measurements, and data fitting are described in Methods. The difference between the best fit and experimental data (χ2, see Methods) are shown for each metabolite and summarized for the whole set of data.

Marin de Mas et al. BMC Systems Biology 2011 5:175   doi:10.1186/1752-0509-5-175

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