Open Access Methodology article

Compartmentation of glycogen metabolism revealed from 13C isotopologue distributions

Igor Marin de Mas12, Vitaly A Selivanov123*, Silvia Marin12, Josep Roca4, Matej Orešič5, Loranne Agius6 and Marta Cascante12*

Author Affiliations

1 Department of Biochemistry and Molecular Biology, Faculty of Biology, Universitat de Barcelona, Av Diagonal 643, 08028 Barcelona, Spain

2 Institute of Biomedicine of Universitat de Barcelona (IBUB) and CSIC-Associated Unit, Spain

3 A.N.Belozersky Institute of Physico-Chemical Biology, MSU, Moscow 199899, Russia

4 Hospital Clínic, IDIBAPS, CIBERES; Universitat de Barcelona, Barcelona 08028, Spain

5 Technical Research Centre of Finland, Espoo, and Institute for Molecular Medicine, Helsinki, Finland

6 Institute of Cellular Medicine, The Medical School, Newcastle University, Newcastle, UK

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BMC Systems Biology 2011, 5:175  doi:10.1186/1752-0509-5-175

Published: 28 October 2011

Additional files

Additional file 1:

Differential equations of the used kinetic models. Kinetic models were used to simulate the total fluxes and concentrations of metabolites. Based on these calculated total values Isodyn further simulates the distribution of isotopic isomers.

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Additional file 2:

Measured distributions of 13C isotopomers in metabolites. The table presents the data for each independent experiment obtained as described in Methods after subtraction natural 13C isotope enrichment.

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