Table 2

Data sets analyzed by RCR for assessment and augmentation of the Cellular Stress Network model

Data Set

Hyperoxia

HOCl

OxPAPC

Hypoxia


Data Set ID

GSE495

GSE15457

GSE20060

GSE11341


PubMed ID

N/A

19376150

20170901

18469115


Perturbation

100% O2

1.4 mM hypochlorous acid

40 μg/ml oxidized phospholipid

1% O2


Tissue/Cells

Whole lung

RAW 267.4 cell line

Aortic endothelial cells

Lung microvascular endothelial cells


In vivo/in vitro

In vivo

In vitro

In vitro

In vitro


Species

Mouse

Mouse

Human

Human


Timepoint

48 h

6 h

4 h

48 h


Control

0 h

6 h untreated

4 h untreated

0 h


Platform

Affymetrix U74v2

Affymetrix 430_2

Affymetrix HGU133A

Affymetrix HGU133A


# State Changes

1122

1618

185

639


Measured outcome(s)

None

Cell viability, RT-PCR, and Western blot analysis of Nrf2 and Nrf2 target genes

qRT-PCR for HMOX1, GJA5

Hypoxia-induced marker genes; Scratch wound assay


Schlage et al. BMC Systems Biology 2011 5:168   doi:10.1186/1752-0509-5-168

Open Data