Simulated consequences of the redox effects of off-target variations in shRNA perturbation. Model predicted dynamics of intracellular H2O2 accumulation (first column), glutathione redox potential (second column), thioredoxin redox potential (third column), and Pr-SSG accumulation (fourth column) after a 100 μM extracellular H2O2 treatment. Solid lines represent the model-predicted behavior for the ideal KD cell lines with no off-target effects (w.t., Grx1, Trx1, G6PD), and dashed lines represent the model-predicted behavior for the experimentally characterized shRNA cell lines with qRT PCR-determined off-target effects (pLKO, Grx1*, Trx1*, G6PD*).
Kippner et al. BMC Systems Biology 2011 5:164 doi:10.1186/1752-0509-5-164