Characterization of shRNA cell lines. (A) β-Actin normalized mRNA levels of target silencing in selected cell lines with respect to pLKO. (B) Expression levels of target protein after stable shRNA silencing of Grx1, Trx1, and G6PD in Jurkat cells compared to pLKO empty vector control. Selected shRNA sequence is denoted with asterisk. (C) Growth curves of selected stable knockdown cell lines show changes in cell proliferation rates between shRNA and control cells. Error bars represent ± standard error (n = 3).
Kippner et al. BMC Systems Biology 2011 5:164 doi:10.1186/1752-0509-5-164