Table 2

Model-based predictions and experimental data on net CO2 release, biomass yield and RubisCO flux for photoheterotrophic growth of PNSB on different substrates.

Succinate

Malate

Acetate

Fructose

Propionate

Hydrogen (autotroph)



Net CO2 release

Scenario S1/S2

Scenario S1/S2

Scenario S1/S3/S4/S5

Scenario S1/S2

Scenario S1/S2

Scenario S1/S2


calculated [mmol CO2/mmol substrate]

0.72

1.19

0.13

0.38

-0.28

-0.47

([mmol CO2/C in substrate])

(0.181)

(0.300)

(0.064)

(0.064)

(-0.094)

(∞)

measured [mmol CO2/mmol substrate]

0.75 [37]

1.15 [37]

0.11 [10]

-0.23 [37]

0.70 [36]

1.22 [36]

0.25 [37]

0.17 [36]



Biomass yield YX/S

Scenario S1/S2

Scenario S1/S2

Scenario S1/S3/S4/S5

Scenario S1/S2

Scenario S1/S2

Scenario S1/S2


calculated [gDCW/mmol substrate]

0.072

0.062

0.041

0.124

0.072

0.010

calculated [gDCW/g substrate]

0.610

0.463

0.683

0.688

0.973

5.000

measured [gDCW/g substrate]

0.476 [52]

0.660 [44]

0.650[10]



RubisCO flux

Scenario S2

Scenario S2

Scenario S3/S4/S5

Scenario S2

Scenario S2

Scenario S2


Calculated minimal RubisCO flux [mmol/mmol substrate]

0.73

0.20

S3: 0.22

0.40

0.73

0.53

S4: 0.22

S5: 0

[mmol/mmol C in substrate]

(0.184)

(0.05)

S3: 0.11

(0.067)

(0.245)

(∞)

S4: 0.11

S5: 0

Measured [mmol/mmol substrate]

S3: 0.29[10]


The data taken from references [36] and [37] are averaged values over replicated experiments.

Hädicke et al. BMC Systems Biology 2011 5:150   doi:10.1186/1752-0509-5-150

Open Data