Table 5 |
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|
Metabolic fluxes comparison for wild-type and mutant C. glutamicum |
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|
Wildtype |
Mutant |
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|
|
|
||||||||
|
Becker |
OpenFLUX |
FIA |
Becker |
OpenFLUX |
FIA |
||||
|
|
|
||||||||
|
const. |
meas. |
ratios |
const. |
meas. |
|||||
|
|
|||||||||
|
Glucose 6-phosphate isomerase |
49.8 |
51.2 |
51.9 |
52.0 |
51.5 |
41.6 |
40.4 |
42.1 |
42.5 |
|
Glucose 6-phosphate dehydrogenase |
46.8 |
45.0 |
44.7 |
44.7 |
45.1 |
56.2 |
57.5 |
55.7 |
55.1 |
|
Transaldolase |
14 |
13.4 |
13.3 |
13.3 |
13.4 |
17.5 |
17.7 |
17.3 |
17.0 |
|
Transketolase 1 |
14 |
13.4 |
13.3 |
13.3 |
13.4 |
17.5 |
17.7 |
17.3 |
17.0 |
|
Transketolase 2 |
11.9 |
11.3 |
11.2 |
11.2 |
11.3 |
15.8 |
16.4 |
15.6 |
15.4 |
|
Glyceraldehyde 3-phosphate dehydrogenase |
157.5 |
158.0 |
158.2 |
158.6 |
158.0 |
160.8 |
161.0 |
161.0 |
160.5 |
|
Pyruvate kinase |
147.3 |
148.0 |
147.8 |
148.2 |
147.6 |
152.6 |
152.0 |
152.5 |
152.0 |
|
Pyruvate dehydrogenase |
77.5 |
75.8 |
74.8 |
74.9 |
74.9 |
87.5 |
85.2 |
85.1 |
79.7 |
|
Pyruvate carboxylase - carboxykinase |
34.4 |
35.8 |
35.9 |
36.1 |
35.8 |
31.5 |
32.4 |
32.5 |
34.9 |
|
Citrate synthase |
52.5 |
50.8 |
49.6 |
49.7 |
49.9 |
67.7 |
65.4 |
65.3 |
58.9 |
|
Isocitrate dehydrogenase |
52.5 |
50.8 |
49.6 |
49.7 |
49.9 |
67.7 |
65.4 |
65.3 |
58.9 |
|
Oxoglutarate dehydrogenase |
41.2 |
39.4 |
38.2 |
38.3 |
38.5 |
59.9 |
57.6 |
57.5 |
50.7 |
|
Aspartokinase |
11.2 |
11.2 |
11.2 |
11.4 |
11.2 |
14.2 |
14.2 |
14.2 |
15.9 |
|
|
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|
Estimated metabolic fluxes values for the different approaches - the ad-hoc simulation results from Becker et al. [18], the OpenFLUX results [15], and the FIA results for its various simulated scenarios (measured fluxes used as constants, as measurements, and when using ratios of non-normalized data.) |
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|
Srour et al. BMC Systems Biology 2011 5:129 doi:10.1186/1752-0509-5-129 |
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