Figure 3.

Flowchart for ranking pathways. In order to rank pathways, each reaction r = 1 ... N in the enumerated pathways p1 ... pM is first tested for thermodynamical feasibility; enzyme candidates are subsequently tested for performance and homogeneity so that the one with the lowest cost is selected; the cost of toxicity of each reaction product is then added; finally, the nominal flux is estimated for the overall pathway.

Carbonell et al. BMC Systems Biology 2011 5:122   doi:10.1186/1752-0509-5-122
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