Open Access Research article

An Integrated Mathematical Model of Thrombin-, Histamine-and VEGF-Mediated Signalling in Endothelial Permeability

XN Wei123, BC Han13, JX Zhang3, XH Liu3, CY Tan2, YY Jiang2, BC Low14, B Tidor156 and YZ Chen123*

Author Affiliations

1 Computation and Systems Biology, Singapore-MIT Alliance, National University of Singapore, E4-04-10, 4 Engineering Drive 3, 117576, Singapore

2 The Guangdong Provincial Key Laboratory of Chemical Biology, The Graduate School at Shenzhen, Tsinghua University, Shenzhen, China

3 Bioinformatics and Drug Design Group, Department of Pharmacy and Center of Computational Science and Engineering, National University of Singapore, Blk S16, Level 8, 3 Science Drive, 117543, Singapore

4 Research Centre of Excellence in Mechanobiology Institute, Cell Signalling and Developmental Biology Laboratory, Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, 117543, Singapore

5 Department of Biological Engineering, Department of Electrical Engineering & Computer Science, Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology, Room 32-212, Cambridge, MA 02139-4307 USA

6 Singapore-MIT Alliance for Research and Technology, Blk S16, Level 7, 3 Science Drive 2, 117543, Singapore

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BMC Systems Biology 2011, 5:112  doi:10.1186/1752-0509-5-112

Published: 15 July 2011

Additional files

Additional file 1:

Supplementary Figures. Detailed pathway map (Figure S1-S3); Simulated time course and experimental data(Figure S4, S6, S9);Simulated time course of MLC activation in terms of different components (Figure S5, S7, S8, S10-S13); Fit to experimental data for Ras activation (Figure S14); Parameter sensitivity analysis (Figure S15).

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Additional file 2:

Supplementary Table. Chemical reactions and related kinetic parameters used in this model.

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Additional file 3:

Supplementary Model Description. Detail description of signaling cascades used in this model.

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Additional file 4:

SBML file for the model. A Systems Biology Markup Language (SBML) version of our model was provided in Additional File 5.

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Additional file 5:

Sensitivity analysis data. The sensitivity value of ppMLC with respect to all parameters in the model was provided in Additional File 4.

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