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Open Access Research article

Genome-scale reconstruction and in silico analysis of the Ralstonia eutropha H16 for polyhydroxyalkanoate synthesis, lithoautotrophic growth, and 2-methyl citric acid production

Jong Myoung Park12, Tae Yong Kim13 and Sang Yup Lee123*

Author Affiliations

1 Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea

2 BioProcess Engineering Research Center, and Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea

3 Bioinformatics Research Center, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea

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BMC Systems Biology 2011, 5:101  doi:10.1186/1752-0509-5-101

Published: 28 June 2011

Additional files

Additional file 1:

List of metabolic reactions in the genome-scale metabolic model of Ralstonia eutropha H16

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Open Data

Additional file 2:

List of metabolites in the genome-scale metabolic model of Ralstonia eutropha H16

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Open Data

Additional file 3:

Biomass composition of Ralstonia eutropha H16

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Open Data

Additional file 4:

Batch and chemostat culture profile of Ralstonia eutropha H16 in minimal medium

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Additional file 5:

HPLC and GC analysis results in culture of Ralstonia eutropha H16 in minimal medium

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Open Data

Additional file 6:

Carbon source utilization of Ralstonia eutropha H16

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Additional file 7:

mol files for each metabolite to estimate the charges of metabolites for pH 6, 7, and 8 using MarvinBeans software

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Open Data